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<title>UTas ePrints - Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters, the Striped Trumpeter (Latris lineata)</title>
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<meta content="Bransden, M.P." name="eprints.creators_name" />
<meta content="Dunstan, Graeme A." name="eprints.creators_name" />
<meta content="Battaglene, Stephen C." name="eprints.creators_name" />
<meta content="Cobcroft, J.M." name="eprints.creators_name" />
<meta content="Morehead, David T." name="eprints.creators_name" />
<meta content="Kolkovski, S" name="eprints.creators_name" />
<meta content="Nichols, Peter D." name="eprints.creators_name" />
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<meta content="" name="eprints.creators_id" />
<meta content="Stephen.Battaglene@utas.edu" name="eprints.creators_id" />
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<meta content="2007-11-12 03:25:37" name="eprints.datestamp" />
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<meta content="Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3,
22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters,
the Striped Trumpeter (Latris lineata)" name="eprints.title" />
<meta content="pub" name="eprints.ispublished" />
<meta content="300000" name="eprints.subjects" />
<meta content="restricted" name="eprints.full_text_status" />
<meta content="The original publication is available at www.springerlink.com
" name="eprints.note" />
<meta content="We determined the effect of dietary long-chain
(≥C20) PUFA (LC-PUFA), 20:5n-3 and 22:6n-3, on larval striped
trumpeter (Latris lineata) biochemistry through early development
and during live feeding with rotifers (Brachionus plicatilis). Rotifers
were enriched using seven experimental emulsions formulated
with increasing concentrations of n-3 LC-PUFA, mainly
20:5n-3 and 22:6n-3. Enriched rotifer n-3 LC-PUFA concentrations
ranged from 10–30 mg/g dry matter. Enriched rotifers were
fed to striped trumpeter larvae from 5 to 18 d post-hatch (dph) in
a short-term experiment to minimize gross deficiency symptoms
such as poor survival that could confound results. No relationships
were observed between larval growth or survival with dietary
n-3 LC-PUFA at 18 dph. The larval FA profiles generally reflected
those of the rotifer diet, and significant positive regressions
were observed between most dietary and larval FA at 10, 14, and
18 dph. The major exception observed was an inverse relationship
between dietary and larval 22:5n-3. The presence of 22:5n-3
in elevated amounts when dietary 22:6n-3 was depressed suggests
that elongation of 20:5n-3 may be occurring in an attempt
to raise body concentrations of 22:6n-3. We hypothesize that accumulation
of 22:5n-3 might be an early indicator of 22:6n-3 deficiency
in larval fish that precedes a reduction in growth or survival.
A possible role of 22:5n-3 as a biochemical surrogate for
22:6n-3 is discussed." name="eprints.abstract" />
<meta content="2004" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Lipids" name="eprints.publication" />
<meta content="39" name="eprints.volume" />
<meta content="3" name="eprints.number" />
<meta content="215-222" name="eprints.pagerange" />
<meta content="10.1007/s11745-004-1222-6" name="eprints.id_number" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0024-4201" name="eprints.issn" />
<meta content="http://dx.doi.org/10.1007/s11745-004-1222-6" name="eprints.official_url" />
<meta content="1. Sargent, J.R., Tocher, D.R., and Bell, J.G. (2002) The Lipids, in
Fish Nutrition (Halver, J.E., and Hardy, R.W., eds.), pp. 181–257,
Academic Press, San Diego.
2. Tocher, D.R. (2003) Metabolism and Functions of Lipids and
Fatty Acids in Teleost Fish, Rev. Fish. Sci. 2, 107–184.
3. Izquierdo, M.S. (1996) Essential Fatty Acid Requirements of
Cultured Marine Fish Larvae, Aquacult. Nutr. 2, 183–191.
4. Tuncer, H., and Harrell, R.M. (1992) Essential Fatty Acid Nutrition
of Larval Striped Bass (Morone saxatilis) and Palmetto
Bass (M. saxatilis × M. chrysops), Aquaculture 101, 105–121.
5. Salhi, M., Izquierdo, M.S., Hernández-Cruz, C.M., González,
M., and Fernández-Palacios, H. (1994). Effect of Lipid and n-3
HUFA Levels in Microdiets on Growth, Survival, and Fatty
Acid Composition of Larval Gilthead Sea Bream (Sparus aurata),
Aquaculture 124, 275–282.
6. Dhert, P., Lavens, P., Duray, M., and Sorgeloos, P. (1990) Improved
Larval Survival at Metamorphosis of Asian Seabass
(Lates calcarifer) Using ω-3-HUFA-Enriched Live Food, Aquaculture
90, 63–74.
7. Sargent, J.R., Bell, J.G., Bell, M.V., Henderson, R.J., and
Tocher, D.R. (1995) Requirement Criteria for Essential Fatty
Acids, J. Appl. Ichthyol. 11, 183–198.
8. Sargent, J., Henderson, R.J., and Tocher, D.R. (1989) The
Lipids, in Fish Nutrition (Halver, J.E., ed.), pp. 153–218, Academic
Press, San Diego.
9. Bell, M.V., Batty, R.S., Dick, J.R., Fretwell, K., Navarro, J.C.,
and Sargent, J.R. (1995) Dietary Deficiency of Docosahexaenoic
Acid Impairs Vision at Low Light Intensities in Juvenile
Herring (Clupea harengus L.), Lipids 30, 443–449.
10. Morehead, D.T., Ritar, A.J., and Pankhurst, N.W. (2000) Effect
of Consecutive 9- or 12-Month Photothermal Cycles and Handling
on Sex Steroid Levels, Oocyte Development, and Reproductive
Performance in Female Striped Trumpeter Latris lineata
(Latrididae), Aquaculture 189, 293–305.
11. Trotter, A.J., Battaglene, S.C., and Pankhurst, P.M. (2003) Effects
of Photoperiod and Light Intensity on Initial Swim Bladder
Inflation, Growth, and Post-inflation Viability in Cultured
Striped Trumpeter (Latris lineata) Larvae, Aquaculture 224,
141–158.
12. Brown, M.R., Skabo, S., and Wilkinson, B. (1998) The Enrichment
and Retention of Ascorbic Acid in Rotifers Fed Microalgal
Diets, Aquacult. Nutr. 4, 151–156.
13. Lewis, T., Nichols, P.D., and McMeekin, T.A. (2000) Evaluation
of Extraction Methods for Recovery of Fatty Acids from
Lipid-Producing Microheterotrophs, J. Microbiol. Methods 43,
107–116.
14. Bligh, E.G., and Dyer, W.G. (1959) A Rapid Method of Total
Lipid Extraction and Purification, Can. J. Biochem. Physiol. 37,
911–917.
15. Koven, W., Barr, Y., Lutzky, S., Ben-Atia, I., Weiss, R., Harel,
M., Behrens, P., and Tandler, A. (2001) The Effect of Dietary
Arachidonic Acid (20:4n-6) on Growth, Survival and Resistance
to Handling Stress in Gilthead Seabream (Sparus aurata) Larvae,
Aquaculture 193, 107–122.
16. Mourente, G., Rodriguez, A., Tocher, D.R., and Sargent, J.R.
(1993) Effects of Dietary Docosahexaenoic Acid (DHA, 22:6n-3)
on Lipid and Fatty Acid Compositions and Growth in Gilthead
Sea Bream (Sparus aurata L.) Larvae During First Feeding,
Aquaculture 112, 79–98.
17. Rodriguez, C., Perez, J.A., Izquierdo, M.S., Mora, J., Lorenzo,
A., Fernandez-Palacios, H. (1994) Essential Fatty Acid Requirements
of Larval Gilthead Sea Bream, Sparus aurata (L.),
Aquacult. Fish. Manage. 25, 295–304.
18. Furuita, H., Konishi, K., and Takeuchi, T. (1999) Effect of Different
Levels of Eicosapentaenoic Acid and Docosahexaenoic
Acid in Artemia nauplii on Growth, Survival, and Salinity Tolerance
of Larvae of the Japanese Flounder, Paralichthys olivaceus,
Aquaculture 170, 59–69.
19. Izquierdo, M.S., Arakawa, T., Takeuchi, T., Haroun, R., and
Watanabe, T. (1992) Effect of n-3 HUFA Levels in Artemia on
Growth of Larval Japanese Flounder (Paralichthys olivaceus),
Aquaculture 105, 73–82.
20. Harel, M., Lund, E., Gavasso, S., Herbert, R., and Place, A.R.
(2000) Modulation of Arachidonate and Docosahexaenoate in
Morone chrysops Larval Tissues and the Effect on Growth and
Survival, Lipids 35, 1269–1280.
21. Ruyter, B., and Thomassen, M.S. (1999) Metabolism of n-3 and
n-6 Fatty Acids in Atlantic Salmon Liver: Stimulation by Essential
Fatty Acid Deficiency, Lipids 34, 1167–1176.
" name="eprints.referencetext" />
<meta content="Bransden, M.P. and Dunstan, Graeme A. and Battaglene, Stephen C. and Cobcroft, J.M. and Morehead, David T. and Kolkovski, S and Nichols, Peter D. (2004) Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters, the Striped Trumpeter (Latris lineata). Lipids, 39 (3). pp. 215-222. ISSN 0024-4201" name="eprints.citation" />
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<link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" />
<meta content="Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3,
22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters,
the Striped Trumpeter (Latris lineata)" name="DC.title" />
<meta content="Bransden, M.P." name="DC.creator" />
<meta content="Dunstan, Graeme A." name="DC.creator" />
<meta content="Battaglene, Stephen C." name="DC.creator" />
<meta content="Cobcroft, J.M." name="DC.creator" />
<meta content="Morehead, David T." name="DC.creator" />
<meta content="Kolkovski, S" name="DC.creator" />
<meta content="Nichols, Peter D." name="DC.creator" />
<meta content="300000 Agricultural, Veterinary and Environmental Sciences" name="DC.subject" />
<meta content="We determined the effect of dietary long-chain
(≥C20) PUFA (LC-PUFA), 20:5n-3 and 22:6n-3, on larval striped
trumpeter (Latris lineata) biochemistry through early development
and during live feeding with rotifers (Brachionus plicatilis). Rotifers
were enriched using seven experimental emulsions formulated
with increasing concentrations of n-3 LC-PUFA, mainly
20:5n-3 and 22:6n-3. Enriched rotifer n-3 LC-PUFA concentrations
ranged from 10–30 mg/g dry matter. Enriched rotifers were
fed to striped trumpeter larvae from 5 to 18 d post-hatch (dph) in
a short-term experiment to minimize gross deficiency symptoms
such as poor survival that could confound results. No relationships
were observed between larval growth or survival with dietary
n-3 LC-PUFA at 18 dph. The larval FA profiles generally reflected
those of the rotifer diet, and significant positive regressions
were observed between most dietary and larval FA at 10, 14, and
18 dph. The major exception observed was an inverse relationship
between dietary and larval 22:5n-3. The presence of 22:5n-3
in elevated amounts when dietary 22:6n-3 was depressed suggests
that elongation of 20:5n-3 may be occurring in an attempt
to raise body concentrations of 22:6n-3. We hypothesize that accumulation
of 22:5n-3 might be an early indicator of 22:6n-3 deficiency
in larval fish that precedes a reduction in growth or survival.
A possible role of 22:5n-3 as a biochemical surrogate for
22:6n-3 is discussed." name="DC.description" />
<meta content="2004" name="DC.date" />
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<meta content="Bransden, M.P. and Dunstan, Graeme A. and Battaglene, Stephen C. and Cobcroft, J.M. and Morehead, David T. and Kolkovski, S and Nichols, Peter D. (2004) Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters, the Striped Trumpeter (Latris lineata). Lipids, 39 (3). pp. 215-222. ISSN 0024-4201" name="DC.identifier" />
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<h1 class="ep_tm_pagetitle">Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters, the Striped Trumpeter (Latris lineata)</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Bransden, M.P.</span> and <span class="person_name">Dunstan, Graeme A.</span> and <span class="person_name">Battaglene, Stephen C.</span> and <span class="person_name">Cobcroft, J.M.</span> and <span class="person_name">Morehead, David T.</span> and <span class="person_name">Kolkovski, S</span> and <span class="person_name">Nichols, Peter D.</span> (2004) <xhtml:em>Influences of Dietary n-3 Long Chain PUFA on Body Concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in Larvae of a Marine Teleost Fish from Australian Waters, the Striped Trumpeter (Latris lineata).</xhtml:em> Lipids, 39 (3). pp. 215-222. ISSN 0024-4201</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2437/1/Influences_lipids.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2437/1/Influences_lipids.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />121Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3189" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1007/s11745-004-1222-6">http://dx.doi.org/10.1007/s11745-004-1222-6</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">We determined the effect of dietary long-chain
(≥C20) PUFA (LC-PUFA), 20:5n-3 and 22:6n-3, on larval striped
trumpeter (Latris lineata) biochemistry through early development
and during live feeding with rotifers (Brachionus plicatilis). Rotifers
were enriched using seven experimental emulsions formulated
with increasing concentrations of n-3 LC-PUFA, mainly
20:5n-3 and 22:6n-3. Enriched rotifer n-3 LC-PUFA concentrations
ranged from 10–30 mg/g dry matter. Enriched rotifers were
fed to striped trumpeter larvae from 5 to 18 d post-hatch (dph) in
a short-term experiment to minimize gross deficiency symptoms
such as poor survival that could confound results. No relationships
were observed between larval growth or survival with dietary
n-3 LC-PUFA at 18 dph. The larval FA profiles generally reflected
those of the rotifer diet, and significant positive regressions
were observed between most dietary and larval FA at 10, 14, and
18 dph. The major exception observed was an inverse relationship
between dietary and larval 22:5n-3. The presence of 22:5n-3
in elevated amounts when dietary 22:6n-3 was depressed suggests
that elongation of 20:5n-3 may be occurring in an attempt
to raise body concentrations of 22:6n-3. We hypothesize that accumulation
of 22:5n-3 might be an early indicator of 22:6n-3 deficiency
in larval fish that precedes a reduction in growth or survival.
A possible role of 22:5n-3 as a biochemical surrogate for
22:6n-3 is discussed.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The original publication is available at www.springerlink.com
</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300000.html">300000 Agricultural, Veterinary and Environmental Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2437</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">12 Nov 2007 14:25</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2437;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2437">item control page</a></p>
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